Emerging Model Organisms by Wei Wang, Nicolas Rohner, Yongfu Wang
English | PDF,EPUB | 2023 | 361 Pages | ISBN : 1071628747 | 63.6 MB
This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging, Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.