Manual of Diagnostic Antibodies for Immunohistology

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Manual of Diagnostic Antibodies for Immunohistology


Anthony S-Y Leong, «Manual of Diagnostic Antibodies for Immunohistology»
Humana Press | ISBN 1588291480 | 2003 Year | PDF | 1 Mb | 192 Pages



Summary:
The rapid acceptance of immunohistochemistry as an important and even indispensable adjunct to morphological examination and diagnosis requires the modern anatomical pathology laboratory to be conversant with, and proficient in, immunostaining procedures, as well as methods of tissue processing and antigen retrieval and the underlying characteristics of the increasing number of antibodies and antisera available. This fully revised, expanded and updated edition provides a comprehensive list of antisera and monoclonal antibodies that have useful diagnostic applications in tissue sections and cell preparations. Various clones, which are commercially available to detect the same antigen, are listed and the sensitivities and specificities of the antibodies are discussed. Importantly, the authors, all pathologists with a wealth of experience in immunostaining procedures, provide fully referenced details and expert advice on each reagent, which the reader will find invaluable. Finally, the appendices provide easily accessible and clear summaries of selected antibody panels for specific diagnostic situations, details of heat-induced antigen/epitope retrieval (including the use of microwaves) and a useful reference to the websites of the main antibody suppliers.

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