Handbook of Single-Molecule Biophysics (Repost)
Peter Hinterdorfer, "Handbook of Single-Molecule Biophysics"
English | 2009 | ISBN: 0387764968 | DJVU | pages: 634 | 14,2 mb
English | 2009 | ISBN: 0387764968 | DJVU | pages: 634 | 14,2 mb
This book will introduce the reader to the different classes of single-molecule approaches developed over recent years and how these methods can be applied to understand biological systems. Broadly, single-molecule biophysical techniques can be separated into two classes of methods: one that allows us to visualize single molecules, the other to manipulate them. The potential of imaging the emission of single fluorophores has led to many exciting developments that allow us to study biomacromolecules, their movement, their interactions, and their cellular context. Biochemical and genetic methods have been developed that allow the very specific and precise fluorescent labeling of biomacromolecules. Optical excitation and detection methods have been introduced that allow the visualization of individual, fluorescently labeled molecules with high spatial and temporal resolution.
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