"Cryopreservation in Eukaryotes" ed. by Francisco Marco-Jiménez and Hülya Akdemi

This volume is a comprehensive collection of the most frequently used methods for eukaryotes. With this book, every researcher will better understand the principles, background, and current status of cryopreservation in particular organisms.

Since accidentally discovering the ability of glycerol on protecting cells from freezing damage, many researchers have been pursuing to develop cryopreservation methods of a very wide range of cells and some tissues, and these have found widespread applications in biology and medicine. From the point of view of living organisms, cryopreservation is a useful tool for ex situ conservation of genetic resources together with its contribution on conservation of their biodiversity.

Contents
Preface
1 Isolation and Cryopreservation of Trypanosomes and their Vectors for Research and Development in Resource‐ Constrained Settings
2 Microscopic Evaluation of Necrotic Cell Death in the Cartilage Destined for Experimental Tracheal Allografts: Lyophilization vs Cryopreservation
3 Isolation and Cryopreservation of Animal Mesenchymal Stromal Cells
4 Semen Cryopreservation in Brazilian Freshwater Fish: Advances and Main Challenges
5 Cryopreservation of Nile Tilapia (Oreochromis niloticus) Sperm
6 Long‐Term Storing of Frozen Semen at −196°C does not Affect the Post-Thaw Sperm Quality of Bull Semen
7 The Roles of Antioxidants and Fatty Acids in Sperm Cryopreservation
8 Cryopreservation of Epididymal Sperm
9 Cryopreservation of Embryos and Gametes: Past, Present, and Future
10 Cryopreservation of Human Gametes and Embryos: Current State and Future Perspectives
11 Quality Control Factors Influencing the Successful and Reliable Implementation of Oocyte and Embryo Vitrification
12 Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana