Two-Hybrid Systems: Methods and Protocols (Methods in Molecular Biology) by Paul N. MacDonald
English | May 17, 2001 | ISBN: 0896038327, 1617372102, 0896038084 | 350 Pages | PDF | 3 MB
English | May 17, 2001 | ISBN: 0896038327, 1617372102, 0896038084 | 350 Pages | PDF | 3 MB
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast), to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems).