Guanylate Cyclase and Cyclic GMP: Methods and Protocols
Thomas Krieg, Robert Lukowski, "Guanylate Cyclase and Cyclic GMP: Methods and Protocols"
English | ISBN: 1627034587 | 2013 | PDF | 165 pages | 4,5 MB
English | ISBN: 1627034587 | 2013 | PDF | 165 pages | 4,5 MB
Recently, many ground-breaking steps have been made towards better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.